Ideal Purity Levels of Recombinant Proteins for Different Applications
From drug development to biological research, recombinant proteins have become one of the most important parts of scientific research.
In fact, the market for recombinant proteins is expected to exceed $3.2B by 2028, highlighting their importance in both research and clinical settings.
With that said, selecting the right purity level of recombinant proteins is crucial to achieve accurate results, and this process is not easy.
This is why we will discuss the ideal purity levels for various applications to help you make better decisions when it comes to selecting the right purity level of recombinant proteins to achieve reliable and effective results.
1. Purity Level for Basic Research
ELISA and Western blotting are two fundamental techniques in basic research to detect and quantify proteins.
These techniques use recombinant proteins with a purity level (85%-95%), including monoclonal recombinant antibodies, to study:
-
Protein functions
-
Expression patterns
-
Modifications
NOTE: When studying protein interactions, you need high accuracy. So, you should consider proteins with a purity level of >95%.
Recombinant proteins with a purity level of >95% for high-accuracy research:
-
Recombinant Human Centromere Protein-B (Purity: >95%)
-
Recombinant Human High-Mobility Group Box 1 (Purity: >95%)
-
Recombinant Human Retinoblastoma Associated Protein (Purity: >95%)
-
Recombinant Human DNA Topoisomerase-I (Purity: >95%)
2. Purity Level for Functional Assays
In functional assays, recombinant proteins are used to study:
-
How proteins work
-
How they interact with other molecules
-
How enzymes behave
These tests help to understand protein functions and disease processes, like how proteins interact with substrates or catalyze chemical reactions.
To get accurate results, the purity level of the recombinant proteins needs to be >95%. This ensures that impurities from other proteins or contaminants dont affect the test results. In more specialized tests, like drug discovery screenings or enzyme studies, proteins with >99% purity are needed.
Recombinant proteins for functional assays with purity >95%:
-
Recombinant Human Aminoadipate-Semialdehyde Dehydrogenase (Purity: >95%)
-
Recombinant Human Stanniocalcin-1 (Purity: >95%)
-
Recombinant Human Mammaglobin-A (Purity: >95%)
3. Purity Level for Therapeutic Application
These proteins are used in various therapeutic applications:
-
Monoclonal antibodies
-
Hormone treatments
-
Enzyme replacement therapies
They need to be very pure to avoid any contaminants like host cell proteins, DNA, or toxins, which could trigger immune reactions, and cause harmful side effects. So, consider a recombinant protein with purity level >99% to avoid any risks.
Recombinant proteins for therapeutic applications:
-
Recombinant Hepatitis B Surface Antigen (HBsAg) (Purity: >99%)
4. Diagnostic Applications
Recombinant proteins are used to find and measure specific molecules, like biomarkers for diseases or infections, in diagnostic tests. To get an accurate results, 90% purity level is sufficient enough as long as the test is designed correctly. For tests which need high specificity to detect small amounts of biomarkers, then a purity level of >90% may be required.
Recombinant proteins with a purity level of >90%:
-
Recombinant Human Vascular Endothelial Growth Factor C (VEGFC) (Purity: >97%)
-
Recombinant Human Fibroblast Growth Factor 15 (FGF15) (Purity: >95%)
The Bottom Line
Now that you understand the ideal purity level for recombinant proteins, you can make sure to get accurate results and successful outcomes in your experiments or clinical applications. Look for high-quality proteins from a trusted source like AAA Biotech. Just keep in mind to check purity standards and quality assurance of the proteins.
